Anti HBc IGM ELISA kit

Product name: Human IgM ELISA Kit

Detection method: Colorimetric

Example type: Cell culture supernatant, Saliva, Milk, Serum, Hep Plasma, EDTA Plasma, Cit Plasma

Test type: Sandwich (quantitative)

Sensitivity: 50 pg / ml

Distance: 390.63 pg / ml – 25000 pg / ml

Test time: 1h 30m

Trial duration: One-step test

Species reactivity:

Reacts with: Human
Does not react with: Goat, Cow

Product description

The Human IgM ELISA Kit (ab214568) is a 90-minute single wash sandwich ELISA designed for the quantitative measurement of IgM protein in the cell culture supernatant, cit plasma, EDTA plasma, hep plasma, milk, saliva, and serum. It uses our patented SimpleStep ELISA technology. Quantify human IgM with a sensitivity of 50 pg/ml. SimpleStep ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA plates. This sandwich ELISA approach allows for the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the simple steps ELISA protocol summary in the imaging section for more details. Our SimpleStep ELISA technology offers several benefits:

– Single wash protocol reduces assay time to 90 minutes or less
– High sensitivity, specificity, and reproducibility of superior antibodies
– Fully validated in biological samples
– 96-well plate that can be broken into 12 x 8-well strips

Calibration

This immunoassay is calibrated against highly purified human IgA. The NIBSC / OMS 67/086 purified unclassified human IgG / IgM / IgG preparation. was evaluated in this kit. The dose-response curve of the unclassified standard parallels the SimpleStep standard curve. To convert the sample values ​​obtained with the SimpleStep IgA kit to approximate NIBSC International units, use the following equation. Approximate NIBSC 67/086 value (IU / ml) = 0.000249 (2.5e-4 IU) x IgA SimpleStep value (ng / ml)

Notes

Immunoglobulin M, or IgM, is a basic antibody produced by B cells and the largest of the immunoglobulins in the human circulatory system. IgM typically constitutes about 10% of serum immunoglobulins and is prominent in early immune responses. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. IgM typically forms pentamers that include the J chain, while in the less common hexameric form the J chain is absent. Although the common pentameric form of IgM contains 10 antigen-binding sites, IgM cannot bind 10 antigens simultaneously due to size limitations.

Class-specific anti-immunoglobulin antibodies are useful for the characterization of malignant B cell proliferations. Most lymphocytic leukemias share surface or intracytoplasmic Ig with an isotypic restriction, suggesting the monoclonal nature of the cell population. Most chronic lymphocytic leukemias, non-Hodgkin’s lymphomas, and Burkitt’s lymphoma carry surface IgM, whereas plasma cells in Waldenström’s disease carry intracytoplasmic IgM.

Author: Justin

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