Designed for the quick and convenient extraction of LPS from gram-negative bacteria
- Extraction/purification of LPS from gram-negative bacteria
- LPS extraction by the phenol-water method
- Efficient removal of sources of contamination such as proteins and nucleic acids.
- Simple and efficient LPS extraction
- LPS extraction in 60 min
General Description
In general, gram-negative bacteria have a separate structure called lipopolysaccharide (LPS) in addition to the phospholipid and protein in the outer layer of the cell envelope, which has different characteristics depending on the bacteria. Such LPS is known not only to play an important role in the growth or survival of bacteria but also to play many roles, particularly in the interaction between the host and the parasite. Furthermore, it is understood that LPS has a close relationship with the immune reaction, for example, it elicits endotoxin secretion when the septic shock is administered and as a result, causes peripheral vascular collapse and increased LPS is important in the reaction pathophysiological toxic.
Thus, the LPS has a different extraction pattern depending on the bacteria and this also allows the bacteria to be systematically separated. As mentioned above, bacterial endotoxin is a material that is generally present on the outer wall of cells in gram-negative bacteria, which does not appear while the bacteria is alive but, when the bacteria multiply or die, it is released and breaks down cell walls. Natural endotoxin comprises LPS, proteins, and phospholipids, which are very stable, heat resistant, negatively charged, and have a bulky molecular weight (1,000,000 daltons or more).
In general, the protein is obtained from extraction with trichloroacetic acid, butanol, or EDTA and the removal of lipid-associated proteins (LAP), and the phospholipids are extracted with phenol. When we normally try to extract LPS, LPS of about 1 ~ 4% by bacterial dry weight can be obtained. The LPS Extraction Kit is based on the phenol water method so this kit denatures the protein in endotoxin and then removes it by denaturation as a precipitate between the water layer and the phenol layer, in addition, this kit separates lipids as a fraction into phenol / chlorophenic form and as a result, can extract only intensive LPS quickly and easily.
Applications
- Study on the organization and structure of LPS
- Systematic study on bacteria
- Study on antibiotic target
- Study on the design of an LPS inhibitor drug
- Study on the immune reaction of carbohydrate antigen
